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vectashilde r○ mounting medium  (Vector Laboratories)


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    Vector Laboratories vectashilde r○ mounting medium
    Vectashilde R○ Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashilde r○ mounting medium/product/Vector Laboratories
    Average 98 stars, based on 21065 article reviews
    vectashilde r○ mounting medium - by Bioz Stars, 2026-03
    98/100 stars

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    IL-7 co-localizes with the recycling endosome marker, Rab11, in cytokine pulsed lymphocytes. Cytospin preparations of PBMC were immunostained for IL-7 and Rab11 after 1 hour of incubation with rIL-7 (100 ng/ml) as described in the methods. Representative image of one lymphocyte showing (A) IL-7 (red), (B) Rab11 (green), and (C) DAPI (blue). (D) Merged image indicates evidence of co-localization of fluorescent signals. (E) The correlation coefficients (Pearson’s R value, and Spearman’s rank correlation value(ρ) for pixel co-localization of IL-7 and Rab11 signal in 18 cells is shown and plotted from the cell with the least correlation to the cell with the greatest correlation. Cells that were not pulsed with IL-7 and antibody isotype control stains were used as negative controls and did not demonstrate evidence of staining (not shown).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Recycled interleukin-7 can be delivered to neighboring T cells

    doi: 10.4049/jimmunol.1400560

    Figure Lengend Snippet: IL-7 co-localizes with the recycling endosome marker, Rab11, in cytokine pulsed lymphocytes. Cytospin preparations of PBMC were immunostained for IL-7 and Rab11 after 1 hour of incubation with rIL-7 (100 ng/ml) as described in the methods. Representative image of one lymphocyte showing (A) IL-7 (red), (B) Rab11 (green), and (C) DAPI (blue). (D) Merged image indicates evidence of co-localization of fluorescent signals. (E) The correlation coefficients (Pearson’s R value, and Spearman’s rank correlation value(ρ) for pixel co-localization of IL-7 and Rab11 signal in 18 cells is shown and plotted from the cell with the least correlation to the cell with the greatest correlation. Cells that were not pulsed with IL-7 and antibody isotype control stains were used as negative controls and did not demonstrate evidence of staining (not shown).

    Article Snippet: Next, the slides were hard-mounted in Vectashild™ with DAPI and examined by an automated epi-fluorescent microscope (EVOS ® FL, Life Technologies).

    Techniques: Marker, Incubation, Staining